OpenFlow enhances the Quality of Service (QoS) of traditional networks, but it has disadvantage that its network session identification efficiency is low and the network packet forwarding path is poor and so on. On the basis of the current study of the OpenFlow, GPU OpenFlow Massive Data Network Analysis (GOMDI) model was proposed by this paper, through integrating the biological sequence algorithm, GPU parallel computing algorithm and machine learning methods. The network session matching algorithm and path selection algorithm of GOMDI were designed. The experimental results show that the speedup of the GOMDI network session matching algorithm is over 300 higher than the CPU environment in real network, and the network packet loss rate of its path selection algorithm is lower than 5%, the network delay is less than 20ms. Thus, the GOMDI model can effectively improve network performance and meet the needs of the real-time processing for massive information in big data environment.